Reconstitution & Storage Guide

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Most peptides are supplied as a freeze-dried powder. This form improves stability by reducing exposure to moisture, heat, and light during transport and storage. After delivery, peptides should be placed in appropriate storage conditions as soon as possible.

Recommended storage conditions

Short-term storage: Refrigerate at 2–8 °C

Long-term storage: Store at −20 °C

When stored as a dry powder at −20 °C, many peptides remain stable for extended periods (often several years under proper conditions).

Peptides gradually degrade over time due to natural chemical instability. Early degradation may have minimal impact, but continued breakdown can lead to structural changes and reduced effectiveness.

Maintaining low temperatures and minimizing exposure to moisture and light helps slow this process significantly.

Lyophilised (powder form):
Stable for up to ~48 months at −20 °C under optimal conditions

Reconstituted (liquid form):
Typically stable for 2–8 weeks when refrigerated at 2–8 °C

Reconstitution refers to dissolving freeze-dried peptide powder in a suitable sterile solvent before use.

Before starting: Allow both the peptide vial and solvent to reach room temperature.
This helps prevent cloudiness and improves dissolution, as cold temperatures causes the powder to dissolve much slower. This would otherwise increase the risk of clumping or incomplete mixing (white particulates forming).

Freeze-dried vials may contain either positive or negative pressure. Equalising pressure helps prevent loss of material during reconstitution.

Before inserting any needle into the peptide or solvent vial, thoroughly wipe the rubber stopper with a sterile alcohol wipe and allow it to air dry completely. Repeat this step each time the stopper is pierced.

Method:
Draw sterile air into a syringe
Insert the needle into the rubber stopper
Allow pressure to naturally equalise before adding liquid

The most commonly used solvent is bacteriostatic water.
Depending on peptide characteristics, alternatives may include:
Diluted acetic acid solutions for slow-dissolving peptides
More concentrated acetic acid solutions for highly hydrophobic compounds

The choice of solvent should align with the intended research application and peptide solubility.

After adding solvent:
Gently swirl the vial until fully dissolved
Avoid shaking, as excessive agitation may affect molecular stability
Slow and controlled mixing helps preserve peptide integrity.

Using excessive liquid can reduce stability and increase degradation risk.
Typical reconstitution volumes range between: 1 mL to 3 mL (depending on experimental requirements)

In some cases, small visible particles or cloudiness may remain after initial mixing.

If this occurs: Allow the vial to sit undisturbed at room temperature for up to 30 minutes T

his resting period often improves dissolution without the need for additional agitation

If particles are still present after this period, gentle swirling or additional time may help achieve full solubility.

Store the reconstituted vial in a refrigerator at 2–8 °C

Keep the vial tightly sealed at all times to reduce contamination and evaporation

Do not freeze the vial again after reconstitution, as freezing can damage the peptide structure and reduce stability

Under proper refrigeration, most reconstituted peptides remain usable for approximately 2–8 weeks, depending on the compound and storage conditions.

Each time solution is withdrawn from the vial, wipe the rubber stopper with a sterile alcohol wipe and allow it to air dry completely before inserting a needle. This helps reduce the risk of contamination during repeated use.

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